MiSeq Companion

Troubleshooting Cycle 1 Errors on the MiSeq
Best focus not found
Best focus is too near the edge of range
No usable signal found (it is possible clustering has failed)
Through-focus peak did not exceed SNR threshold
Z Motor attempt to move outside soft limits
ThroughFocusScan CoarseFocus failed to find focus, and there is no fallback position

Index

MiSeq Self-Service
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Contents - 1
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Contents - 1 Continued
Contents - 2
Contents - 2 continued
Contents - 3
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Section 1
General Use
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Section 1 contents - Preparing for a run
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Sample sheet templates & maximum read lengths
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Choosing a Loading concentration for Illumina sequencing
Optimal Cluster Density Best Practices
Sequencing: Considerations for Low Diversity Libraries
How to use the Illumina® Sequencing Coverage Calculator
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Section 1 contents - Setting up a run
Preparing reagents and flow cell
Preparing Library and PhiX spike-in
Launching a run with LRM in Win10
How to generate an RFID bypass-code in MyIllumina
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MiSeq instrument maintenance
How to perform washes on the MiSeq
How to perform washes & how to clean Illumina NGS sequencing wash cartridges
How to shut down or powercycle the MiSeq
Run folder sizes and Disk Space on the MiSeq
How to enable Proactive on the MiSeq
Best practices for maintaining the computer on a MiSeq
How to perform a System Check on the MiSeq
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Section 1
MiSeq Troubleshooting
Section 2 contents - Troubleshooting 1
Section 2 contents - Troubleshooting 2
Troubleshooting "Unable to Measure Flow Rate" Errors on the MiSeq
Troubleshooting "PR2 Bottle Not Detected" Error on the MiSeq
Troubleshooting "FocusMirror" Error on the MiSeq
Troubleshooting pump errors on the MiSeq
Troubleshooting Y Motor Errors on the MiSeq
Troubleshooting "Z Motor: Positive soft limit too nonlinear at innermost test point" error on the Mi
Troubleshooting best focus errors occurring mid-run on the MiSeq
Troubleshooting Low Cluster Density on MiSeq
Troubleshooting High Cluster Density on MiSeq
Troubleshooting No Intensity for Index Read on MiSeq
How to access Windows on the MiSeq if there is Black Screen at boot up
Troubleshooting frozen MiSeq Control Software, Black Screen or Blue Screen errors during Sequencing
How to Restart RTA on the MiSeq
Troubleshooting “The process cannot access the file”error on MiSeq
Troubleshooting FPGA RFID ERROR STATUS 2 errors on the MiSeq
Troubleshooting timeout FPGA errors on the MiSeq platform
Troubleshooting MiSeq error “a valid index kit must be provided for the selected library prep kit”
Troubleshooting "Failed to get temperature" warning message on the MiSeq
Troubleshooting "Green LED: Failed polling LED status" error on MiSeq
Troubleshooting MiSeq Washes Taking Extended Time to complete
Troubleshooting Object Reference Error on the MiSeq
Troubleshooting "RFID Cannot Be Written" Error on the MiSeq
Troubleshooting Cycle 1 Errors on the MiSeq
Troubleshooting Sipdown Errors on the MiSeq
Section 3
Additional Resources
Bulletins - 1
Bulletins - 2
Technical & Application Notes - 1
Technical & Application Notes - 2
Webinars & Trainings - 1
Webinars & Trainings - 2
How many cycles of SBS chemistry are in my kit?
Online Tools
Order the Miseq and reagent kits
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Back cover